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pET28b-NmCas9-Flag D16A
(Plasmid #109031)


Item Catalog # Description Quantity Price (USD)
Plasmid 109031 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8512
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    CRISPR-associated endonuclease Cas9
  • Alt name
  • Species
    Neisseria meningitidis
  • Insert Size (bp)
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • Flag Tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-NmCas9-Flag D16A was a gift from Yan Zhang (Addgene plasmid # 109031 ; ; RRID:Addgene_109031)
  • For your References section:

    Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis. Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y. Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15. 10.1016/j.molcel.2018.01.025 PubMed 29456189