pET28b-NmCas9-Flag D16A
(Plasmid
#109031)
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PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 8512
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCRISPR-associated endonuclease Cas9
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Alt nameCas9
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SpeciesNeisseria meningitidis
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Insert Size (bp)3273
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GenBank ID
- Promoter T7
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Tag
/ Fusion Protein
- Flag Tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-NmCas9-Flag D16A was a gift from Yan Zhang (Addgene plasmid # 109031 ; http://n2t.net/addgene:109031 ; RRID:Addgene_109031) -
For your References section:
Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis. Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y. Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15. 10.1016/j.molcel.2018.01.025 PubMed 29456189
Map uploaded by the depositor.
