Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis.
Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y
Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109030pET28b-NmCas9-FlagWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
Loading...
109031pET28b-NmCas9-Flag D16AD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
Loading...
109032pET28b-NmCas9-Flag H588AH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
Loading...
109033pET28b-NmCas9-Flag D16A+H588AD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
Loading...