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Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis.
Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y
Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109030pET28b-NmCas9-FlagWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
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109031pET28b-NmCas9-Flag D16AD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
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109032pET28b-NmCas9-Flag H588AH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
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109033pET28b-NmCas9-Flag D16A+H588AD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
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Recombinant Antibodies from Article