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pET28b-NmCas9-Flag
(Plasmid #109030)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 109030 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8512
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CRISPR-associated endonuclease Cas9
  • Alt name
    Cas9
  • Species
    Neisseria meningitidis
  • Insert Size (bp)
    3273
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • Flag Tag (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-NmCas9-Flag was a gift from Yan Zhang (Addgene plasmid # 109030 ; http://n2t.net/addgene:109030 ; RRID:Addgene_109030)
  • For your References section:

    Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis. Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y. Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15. 10.1016/j.molcel.2018.01.025 PubMed 29456189