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(Plasmid #52635)


Item Catalog # Description Quantity Price (USD)
Plasmid 52635 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7416
  • Total vector size (bp) 8852
  • Modifications to backbone
    Insertion of hEµMAR-enhancer into SFFV-promoter
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    any (like TOP10, XL10-Gold or Stbl)
  • Copy number
    High Copy


  • Gene/Insert name

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Primer-sequences for amplification of hEµMAR were kindly provided by X.M. Luo (California Institute of Technology, Pasadena, CA) and modified to clone into LeGO-iG2. The eGFP cDNA is from Clontech. The lenti backbone is a derivative of pLentiLox3.7 developed at the MIT (

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LeGO-hEµMAR-iG2 was a gift from Boris Fehse (Addgene plasmid # 52635 ; ; RRID:Addgene_52635)
  • For your References section:

    Efficient lentiviral transduction and transgene expression in primary human B cells. Mock U, Thiele R, Uhde A, Fehse B, Horn S. Hum Gene Ther Methods. 2012 Dec;23(6):408-15. doi: 10.1089/hgtb.2012.160. 10.1089/hgtb.2012.160 PubMed 23240650