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GFP-TCF4
(Plasmid #109144)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 109144 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCF7L2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TCF7L2 (a.k.a. TCF-4, TCF4)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PMID: 9065401

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Origin - PMID: 9065401

Note: The TCF7L2 insert contains amino acids 1 through 461 compared to the closest reference sequence, NP_110383.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-TCF4 was a gift from Mariann Bienz (Addgene plasmid # 109144 ; http://n2t.net/addgene:109144 ; RRID:Addgene_109144)
  • For your References section:

    Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function. Townsley FM, Cliffe A, Bienz M. Nat Cell Biol. 2004 Jul;6(7):626-33. doi: 10.1038/ncb1141. Epub 2004 Jun 20. 10.1038/ncb1141 PubMed 15208637