PurposeFor the expression of GFP-TCF in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109144||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTCF7L2 (a.k.a. TCF-4, TCF4)
- Promoter CMV
/ Fusion Protein
- eGFP (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Gene Origin - PMID: 9065401
Note: The TCF7L2 insert contains amino acids 1 through 461 compared to the closest reference sequence, NP_110383.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-TCF4 was a gift from Mariann Bienz (Addgene plasmid # 109144 ; http://n2t.net/addgene:109144 ; RRID:Addgene_109144)
For your References section:Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function. Townsley FM, Cliffe A, Bienz M. Nat Cell Biol. 2004 Jul;6(7):626-33. doi: 10.1038/ncb1141. Epub 2004 Jun 20. 10.1038/ncb1141 PubMed 15208637