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PurposeFor the expression of GFP-TCF in mammalian cells
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Depositing Lab
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Depositing OrganizationMRC Laboratory of Molecular Biology
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 109144-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 109144-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCF7L2
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SpeciesH. sapiens (human)
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Entrez GeneTCF7L2 (a.k.a. TCF-4, TCF4)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPMID: 9065401
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene Origin - PMID: 9065401
Note: The TCF7L2 insert contains amino acids 1 through 461 compared to the closest reference sequence, NP_110383.2.
DNA (Catalog # 109144-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 109144-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-TCF4 was a gift from Mariann Bienz (Addgene plasmid # 109144 ; http://n2t.net/addgene:109144 ; RRID:Addgene_109144) -
For your References section:
Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function. Townsley FM, Cliffe A, Bienz M. Nat Cell Biol. 2004 Jul;6(7):626-33. doi: 10.1038/ncb1141. Epub 2004 Jun 20. 10.1038/ncb1141 PubMed 15208637
