B9L-BirA*
(Plasmid
#109146)
-
PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells
-
Depositing Lab
-
Depositing OrganizationMRC Laboratory of Molecular Biology
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 109146-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 109146-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA5/FRT/TO
-
Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 5100
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameB9L
-
SpeciesM. musculus (mouse)
-
Entrez GeneBcl9l (a.k.a. B9L, BC003321, BCL9-2, DLNB, DLNB11)
- Promoter CMV/TetO2
-
Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- BirA(R118G) (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byPMID: 15574752
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene Origin - PMID: 15574752
Note: The Bcl9I insert contains a V229A mutation compared to the closest reference sequence, NP_084532.2. The depositor has confirmed that this does not impact the function of the plasmid.
DNA (Catalog # 109146-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 109146-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
B9L-BirA* was a gift from Mariann Bienz (Addgene plasmid # 109146 ; http://n2t.net/addgene:109146 ; RRID:Addgene_109146) -
For your References section:
Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9. van Tienen LM, Mieszczanek J, Fiedler M, Rutherford TJ, Bienz M. Elife. 2017 Mar 15;6. doi: 10.7554/eLife.20882. 10.7554/eLife.20882 PubMed 28296634
