B9L-BirA*
(Plasmid #109146)

• Purpose: To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells
• Depositing Lab: Mariann Bienz
• Publication: van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife.20882.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B9L
• Species: M. musculus (mouse)
• Entrez Gene: Bcl9l (a.k.a. B9L, BC003321, BCL9-2, DLNB, DLNB11)
• Promoter: CMV/TetO2
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • BirA(R118G) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PMID: 15574752

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Origin - PMID: 15574752

Note: The Bcl9I insert contains a V229A mutation compared to the closest reference sequence, NP_084532.2. The depositor has confirmed that this does not impact the function of the plasmid.