Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p3x-FLAG-hnRNPF
(Plasmid #21926)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21926 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3xFLAG-CMV-7.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hnRNPF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1248
  • GenBank ID
    NP_001091674
  • Entrez Gene
    HNRNPF (a.k.a. HNRPF, OK/SW-cl.23, mcs94-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3 Flags (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3x-FLAG-hnRNPF was a gift from Mariano Garcia-Blanco (Addgene plasmid # 21926 ; http://n2t.net/addgene:21926 ; RRID:Addgene_21926)
  • For your References section:

    hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. Mauger DM, Lin C, Garcia-Blanco MA. Mol Cell Biol. 2008 Sep . 28(17):5403-19. 10.1128/MCB.00739-08 PubMed 18573884