|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10919||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameneu transforming
SpeciesR. norvegicus (rat)
Insert Size (bp)5000
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
See author's map. Expressed in NIH cells, but pSV2 neuNT expresses better.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSV2 neuT was a gift from Bob Weinberg (Addgene plasmid # 10919 ; http://n2t.net/addgene:10919 ; RRID:Addgene_10919)
For your References section:Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann CI, Hung MC, Weinberg RA. Cell. 1986 Jun 6. 45(5):649-57. 10.1016/0092-8674(86)90779-8 PubMed 2871941
Map uploaded by the depositor.