|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10918||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSV2 neo
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)5000
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSV2 neuNT was a gift from Bob Weinberg (Addgene plasmid # 10918 ; http://n2t.net/addgene:10918 ; RRID:Addgene_10918)
For your References section:Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann CI, Hung MC, Weinberg RA. Cell. 1986 Jun 6. 45(5):649-57. 10.1016/0092-8674(86)90779-8 PubMed 2871941
Map uploaded by the depositor.