pEXL flag Smad3
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10920||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEXL (derived from pEGFP-N1)
Backbone manufacturerEGFP-N1 is from Clontech
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-Forward (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
This is a modified version of pEGFP-N1 where the GFP is replaced with flag-Smad3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEXL flag Smad3 was a gift from Bob Weinberg (Addgene plasmid # 10920 ; http://n2t.net/addgene:10920 ; RRID:Addgene_10920)
For your References section:Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Liu X, Sun Y, Constantinescu SN, Karam E, Weinberg RA, Lodish HF. Proc Natl Acad Sci U S A. 1997 Sep 30. 94(20):10669-74. 10.1073/pnas.94.20.10669 PubMed 9380693