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pEXL flag Smad3
(Plasmid #10920)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10920 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEXL (derived from pEGFP-N1)
  • Backbone manufacturer
    EGFP-N1 is from Clontech
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is a modified version of pEGFP-N1 where the GFP is replaced with flag-Smad3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXL flag Smad3 was a gift from Bob Weinberg (Addgene plasmid # 10920 ; http://n2t.net/addgene:10920 ; RRID:Addgene_10920)
  • For your References section:

    Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Liu X, Sun Y, Constantinescu SN, Karam E, Weinberg RA, Lodish HF. Proc Natl Acad Sci U S A. 1997 Sep 30. 94(20):10669-74. 10.1073/pnas.94.20.10669 PubMed 9380693