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pDJSVT87
(Plasmid #109302)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 109302 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone manufacturer
    EMD Millipore
  • Backbone size (bp) 4008
  • Modifications to backbone
    Removed DNA between NcoI and XhoI sites and replaced with a new sequence.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • OmpA 1-27, 6His (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer ACYCDuetUp1
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDJSVT87 was a gift from Daniel Slade (Addgene plasmid # 109302 ; http://n2t.net/addgene:109302 ; RRID:Addgene_109302)
  • For your References section:

    A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. coli. Casasanta MA, Slade DJ. Methods Mol Biol. 2019;1960:123-138. doi: 10.1007/978-1-4939-9167-9_11. 10.1007/978-1-4939-9167-9_11 PubMed 30798527