mCarmine in pRSETB
(Plasmid
#109484)
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Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 109484 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSETB
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 3600
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Modifications to backbonerecombination sites inserted for seamless ligation cloning
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsuse BL21 for protein production
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCarmine
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SpeciesSynthetic; Entactamaea
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Insert Size (bp)1
- Promoter T7
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Tag
/ Fusion Protein
- N-terminal histidine tag in vector
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer pRSET reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
Depositor Comments
mCarmine is a far red emitting fluorescent protein (excitation: 603; emission: 675)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCarmine in pRSETB was a gift from Oliver Griesbeck (Addgene plasmid # 109484 ; http://n2t.net/addgene:109484 ; RRID:Addgene_109484) -
For your References section:
Imaging-Based Screening Platform Assists Protein Engineering. Fabritius Arne, Ng David, Kist Andreas Michael, Erdogan Mutlu, Portugues Ruban, Grisbeck, Oliver. Cell Chemical Biology, 2018 10.1016/j.chembiol.2018.08.008