pMEV4
(Plasmid
#110539)
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Purpose(Empty Backbone) The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepAW42
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Vector typeSynthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMEV4 was a gift from William B. Whitman (Addgene plasmid # 110539 ; http://n2t.net/addgene:110539 ; RRID:Addgene_110539) -
For your References section:
Engineering the Autotroph Methanococcus maripaludis for Geraniol Production. Lyu Z, Jain R, Smith P, Fetchko T, Yan Y, Whitman WB. ACS Synth Biol. 2016 Jul 15;5(7):577-81. doi: 10.1021/acssynbio.5b00267. Epub 2016 Feb 17. 10.1021/acssynbio.5b00267 PubMed 26886063
Map uploaded by the depositor.
