Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #110544)


Item Catalog # Description Quantity Price (USD)
Plasmid 110544 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3941
  • Total vector size (bp) 6059
  • Modifications to backbone
    Addition of two 1000bp sequences with homology to the Synechocystis sp. PCC6803 genome, allowing chromosomal integration. Introduction of the rhaBAD promoter from E. coli (allowing inducible expression with L-rhamnose). Introduction of a synthetic RBS and yfp as a reporter. Introduction of rhaS gene from E. coli, the transcriptional activator required for rhaBAD function in Synechocystis.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    promoter found 5' end of rhaBAD operon in E. coli
  • Species
    E. coli
  • Insert Size (bp)
  • Promoter N/A

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    enhanced yellow fluorescent protein from A. victoria (BBa_E0030)
  • Species
    A. victoria
  • Insert Size (bp)
  • Promoter PrhaBAD

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
  • Alt name
    transcriptional activator of the rhaBAD promoter in E. coli with native E. coli RBS
  • Species
    E. coli
  • Insert Size (bp)
  • Promoter kanR promoter (upstream of kanR, immediately before rhaS)

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK306 was a gift from John Heap (Addgene plasmid # 110544 ; ; RRID:Addgene_110544)
  • For your References section:

    A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria. Kelly CL, Taylor GM, Hitchcock A, Torres-Mendez A, Heap JT. ACS Synth Biol. 2018 Apr 2. doi: 10.1021/acssynbio.7b00435. 10.1021/acssynbio.7b00435 PubMed 29544054