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pAM4891 (pCV0003)
(Plasmid #120080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120080 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 120080-D50 50 μg of DNA in Tris buffer $495
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DNA 120080-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    RSF1010Y25F_aph1_PconII-GFPmut2
  • Backbone size w/o insert (bp) 7846
  • Modifications to backbone
    Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD047 / CV-RSF1010Y25F: The device carries a broad host range replicon derived from RSF1010. A point mutation in mobA prevent self-mobilization. pCVD003 / CV-aph1: The device confers kanamycin or neomycin (Km/Nm) resistance. pCVD030 / CV-PconII-LTRBS-GFPmut2: Promoter/reporter device where GFPmut2 is driven by a PconII promoter. The RBS and flanking sequences are from the pENTR/SD/D-Topo vector (Life Technologies).
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
  • Species
    Broad range of bacteria
  • Mutation
    mobA Y25F

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM4891 (pCV0003) was a gift from Susan Golden (Addgene plasmid # 120080 ; http://n2t.net/addgene:120080 ; RRID:Addgene_120080)

  • For your References section:

    Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. Nucleic Acids Res. 2014 Jul 29. pii: gku673. 10.1093/nar/gku673 PubMed 25074377
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