Purpose(Empty Backbone) RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70692||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial shuttle vector
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin and Streptomycin
Growth instructionsSpectinomycin and Streptomycin for both E.coli and Cyanobacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL1383a was a gift from Peter Wolk (Addgene plasmid # 70692 ; http://n2t.net/addgene:70692 ; RRID:Addgene_70692)
For your References section:Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120. Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch E. Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17. 10.1007/s00203-007-0276-z PubMed 17639350