pBiFC-PBZ-4A-VC
(Plasmid
#110649)
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Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
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Depositing Lab
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Depositing OrganizationInstitute of Cancer Research
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 110649-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 110649-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBiFC-VC155
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Backbone manufacturerChang-Deng Hu, Addgene plasmid #22011
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePBZ domain from APLF, amino acids 371-451
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Alt nameAprataxin and PNK-like factor
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SpeciesH. sapiens (human)
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MutationC379A, C385A, C421A and C427A
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Entrez GeneAPLF (a.k.a. APFL, C2orf13, PALF, Xip1, ZCCHH1)
- Promoter CMV
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- VC (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 110649-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 110649-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBiFC-PBZ-4A-VC was a gift from Chris Lord (Addgene plasmid # 110649 ; http://n2t.net/addgene:110649 ; RRID:Addgene_110649) -
For your References section:
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ. Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4. 10.1038/s41467-018-04466-4 PubMed 29789535
