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Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ
Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110646pBiFC-PBZ-VNexpresses a PAR-binding (PBZ) domain fused to venus N-terminus
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110647pBiFC-PBZ-4A-VNexpresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding
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110648pBiFC-PBZ-VCexpresses a PAR-binding (PBZ) domain fused to venus C-terminus
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110649pBiFC-PBZ-4A-VCexpresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
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110650pcDNA3-PBZ-mRuby2expresses a PAR-binding (PBZ) domain fused to mRuby2
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110651UPATrap-VC-ORF0transposon containing BiFC VC cassette
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110652UPATrap-VC-ORF1transposon containing BiFC VC cassette
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110653UPATrap-VC-ORF2transposon containing BiFC VC cassette
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Antibodies from Article