Pediculus humanus corporis PINK1 (115 - 575)
(Plasmid #110750)

• Purpose: Bacterial expression of PhPINK1 (biochemistry)
• Depositing Lab: David Komander
• Publication: Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi: 10.1038/nature24645. Epub 2017 Oct 30.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Merck
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Phum_PHUM577390
• Alt name: PINK1
• Species: Pediculus humanus corporis
• Entrez Gene: Phum_PHUM577390 (a.k.a. Phum_PHUM577390)
• Promoter: T7
• Tag / Fusion Protein:
  • N-His6-GST-3C (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AATACGACTCACTATAGGG
• 3′ sequencing primer: GGGTTATGCTAGTTATTGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PhPink1: obtained through gene synthesis
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry