pCS2 you
(Plasmid #11084)

• Depositing Lab: William Talbot
• Publication: Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

Backbone

• Vector backbone: modified pCS2+
• Backbone size w/o insert (bp): 4100
• Vector type: zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: you
• Alt name: scube2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 3400
• GenBank ID: AY741664
• Entrez Gene: scube2 (a.k.a. wu:fc27f08, yo, you)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A modified version of pCS2+ was generated by cloning a 41bp fragment into its EcoRI and XbaI sites, abolishing the original sites and introducing XbaI, SacI, ApaI, PstI, and XhoI sequences in a 5' to 3' orientation with respect to the SP6 promoter.