PurposeTo knockdown Thioredoxin-1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110921||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLKO (puromycin)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTXN (a.k.a. TRDX, TRX, TRX1, Trx80)
- Promoter unknown
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The Rai lab uses XL-10 gold ultracompetent cells as the growth strain, but DH5alpha should work fine too.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:plko-puromycin-shTRX1-259 was a gift from Priyamvada Rai (Addgene plasmid # 110921 ; http://n2t.net/addgene:110921 ; RRID:Addgene_110921)
For your References section:Suppression of thioredoxin-1 induces premature senescence in normal human fibroblasts. Young JJ, Patel A, Rai P. Biochem Biophys Res Commun. 2010 Feb 12;392(3):363-8. doi: 10.1016/j.bbrc.2010.01.026. Epub 2010 Jan 13. 10.1016/j.bbrc.2010.01.026 PubMed 20074557