mTalin2_R7R8
(Plasmid
#111041)
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PurposePlasmid expresses the talin2 rod domains R7 and R8
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET151TOPO
- Backbone size w/o insert (bp) 5362
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1035
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Entrez GeneTln2 (a.k.a. 5730421P04Rik, mKIAA0320)
- Promoter T7
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Tag
/ Fusion Protein
- His Tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTalin2_R7R8 was a gift from Ben Goult (Addgene plasmid # 111041 ; http://n2t.net/addgene:111041 ; RRID:Addgene_111041) -
For your References section:
Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. Bouchet BP, Gough RE, Ammon YC, van de Willige D, Post H, Jacquemet G, Altelaar AM, Heck AJ, Goult BT, Akhmanova A. Elife. 2016 Jul 13;5. doi: 10.7554/eLife.18124. 10.7554/eLife.18124 PubMed 27410476
