pCAG-i53-bpA-EF1BFP
(Plasmid #111145)

• Purpose: Expression of i53 and a BFP reporter in mammalian cells
• Depositing Lab: Ralf Kuehn
• Publication: Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.

Backbone

• Vector backbone: Bluescript
• Total vector size (bp): 7175
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: i53
• Species: Synthetic
• Insert Size (bp): 225
• Mutation: human Ubiquitin, contains 8 point mutations
• GenBank ID: AAA72608.1
• Entrez Gene: mutL (a.k.a. HLAC_RS03120, Hlac_0629)
• Promoter: CAG promoter

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Gene/Insert 2

• Gene/Insert name: BFP
• Species: Synthetic
• Insert Size (bp): 720
• Entrez Gene: BFP
• Promoter: EF1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • pCAG-i53-bpA-EF1BFP.gbk
• A portion of this plasmid was derived from a plasmid made by: Amplified from Addgene plasmid 74939 deposited by Daniel Durocher
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Amplified from Addgene plasmid 74939 deposited by Daniel Durocher.