pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2
(Plasmid
#111259)
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Purposemini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC18T-mini-Tn7T-Gm
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Backbone manufacturerHerbert Schweizer
- Total vector size (bp) 5859
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Vector typeBacterial Expression
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTurquoise2
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SpeciesSynthetic
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MutationmTurquoise2 is codon optimized for expression in P. fluorescens
- Promoter Pc
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene NGS results identified a 963T>A mutation in first FRT site compared to the depositing lab's sequence. The effect of this variant on FLP recombination efficiency is unknown.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2 was a gift from Rosemarie Wilton (Addgene plasmid # 111259 ; http://n2t.net/addgene:111259 ; RRID:Addgene_111259) -
For your References section:
A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of Root Colonizing Pseudomonads. Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, Brennan MB, Kemner KM. Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017. 10.3389/fpls.2017.02242 PubMed 29449848
