A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of Root Colonizing Pseudomonads.
Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, Brennan MB, Kemner KM
Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017. PubMed Article
Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, Brennan MB, Kemner KM
Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108234 | pSW002-Pc | Broad host bacterial expression vector with constitutive Pc promoter. |
| 108235 | pSW002-Pc-mTurquoise2 | Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens. |
| 108236 | pSW002-PpsbA | Broad host bacterial expression vector with constitutive PsbA promoter |
| 108237 | pSW002-PpsbA_mTurquoise2 | Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens. |
| 111248 | pSW002 | Broad host-range bacterial vector |
| 111249 | pSW003 | Broad host-range bacterial vector |
| 111250 | pSW002-Pc-mRuby2 | Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens) |
| 111251 | pSW002-Pc-DsRed-Express2 | Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2 |
| 111252 | pSW002-Pc-E2-Crimson | Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson |
| 111253 | pSW002-Pc-TorA(sp) | Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide |
| 111254 | pSW002-Pc-TorA(sp)-mTurquoise2 | Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm |
| 111255 | pSW002-Pc-TorT(sp) | Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide |
| 111257 | pSW002-PpsbA-DsRed-Express2 | Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2 |
| 111258 | pSW002-PpsbA-E2-Crimson | Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson |
| 111259 | pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2 | mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site |
| 111260 | pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2 | mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site |
| 205016 | pSW002-Pc-mNeonGreen | Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens) |
| 205017 | pSW002-PpsbA-mNeonGreen | Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens) |
| 205018 | pSW003-Pc-mNeonGreen | Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens) |
| 205019 | pSW003-PpsbA-mNeonGreen | Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens) |
| 205020 | pSW002-Pc-TorA(sp)-mNeonGreen | Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm |
| 205021 | pSW002-Pc-TorT(sp)-mNeonGreen | Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm |
| 205022 | pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen | mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site |