|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11129||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI and Hind III (not destroyed)
- 3′ cloning site Sal I and Cla I (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was generated by cloning cyclinD1 cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site CDK4R24C cDNA in which HindIII/ClaI sites were again added by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pbabe-cyclinD1+CDK4R24C was a gift from Christopher Counter (Addgene plasmid # 11129 ; http://n2t.net/addgene:11129 ; RRID:Addgene_11129)
For your References section:A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Kendall SD, Linardic CM, Adam SJ, Counter CM. Cancer Res. 2005 Nov 1. 65(21):9824-8. 10.1158/0008-5472.CAN-05-1543 PubMed 16267004