MAC_N_CDK8
(Plasmid
#111685)
-
PurposeMAC-tagged gene expression
-
Depositing Lab
-
Depositing OrganizationUniversity of Helsinki, Institute of Biotechnology
Located in Finland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 111685-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 111685-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneMAC-tag-N
-
Backbone manufacturerMarkku Varjosalo, Addgene plasmid #108078
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCDK8
-
Alt nameCDK8
-
SpeciesH. sapiens (human)
-
Entrez GeneCDK8 (a.k.a. IDDHBA, K35)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CCSB-DFCI hORFeome collection(51020_G07)
DNA (Catalog # 111685-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 111685-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MAC_N_CDK8 was a gift from Markku Varjosalo (Addgene plasmid # 111685 ; http://n2t.net/addgene:111685 ; RRID:Addgene_111685) -
For your References section:
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, Varjosalo M. Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2. 10.1038/s41467-018-03523-2 PubMed 29568061
