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pGEMT-PT2A-GFP-Tdtomato-iRFP670
(Plasmid #111812)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111812 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 111812-D50 50 μg of DNA in Tris buffer $495
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DNA 111812-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGEMT-easy
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6200
  • Vector type
    cloning intermediate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    700

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer M13 F/T7
  • 3′ sequencing primer P2A-r
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Tdtomato
  • Insert Size (bp)
    1400

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer P2A-f
  • 3′ sequencing primer T2A-r
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    iRFP670
  • Insert Size (bp)
    900

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T2A-f
  • 3′ sequencing primer M13R/SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-PT2A-GFP-Tdtomato-iRFP670 was a gift from Li Qian (Addgene plasmid # 111812 ; http://n2t.net/addgene:111812 ; RRID:Addgene_111812)

  • For your References section:

    Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819
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