Skip to main content
Addgene
Open Menu

ppyCAG_RNaseH1_WKKD
(Plasmid #111905)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111905 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 111905-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 111905-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    ppyCAG
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 7279
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNASEH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    777
  • Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to A, D274 to N
  • GenBank ID
    NM_002936
  • Entrez Gene
    RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ppyCAG_RNaseH1_WKKD was a gift from Xiang-Dong Fu (Addgene plasmid # 111905 ; http://n2t.net/addgene:111905 ; RRID:Addgene_111905)

  • For your References section:

    R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters. Chen L, Chen JY, Zhang X, Gu Y, Xiao R, Shao C, Tang P, Qian H, Luo D, Li H, Zhou Y, Zhang DE, Fu XD. Mol Cell. 2017 Nov 16;68(4):745-757.e5. doi: 10.1016/j.molcel.2017.10.008. Epub 2017 Nov 2. 10.1016/j.molcel.2017.10.008 PubMed 29104020
Related items:
Commonly requested with: