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pJW1661
(Plasmid #112035)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112035 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TIR1
  • Species
    Oryza sativa

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gttccgcgcacatttcc
  • 3′ sequencing primer caagctgtgaccgtctcc
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1661 was a gift from Jonathan Weissman (Addgene plasmid # 112035 ; http://n2t.net/addgene:112035 ; RRID:Addgene_112035)
  • For your References section:

    Defining the physiological role of SRP in protein-targeting efficiency and specificity. Costa EA, Subramanian K, Nunnari J, Weissman JS. Science. 2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607. 10.1126/science.aar3607 PubMed 29348368