PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102883||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6024
- Total vector size (bp) 8711
Vector typeYeast Expression
Selectable markersKanMX4 (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameAuxin Inducible Degron
Insert Size (bp)2689
- Promoter ADH1
/ Fusion Protein
- 3x myc
- Cloning method Unknown
- 5′ sequencing primer GGAGTATTGTGTCATGTTCG
- 3′ sequencing primer GACAGTCACATCATGCC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe OsTIR1 was retrieved from: Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., and Kanemaki, M. (2009). An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat. Methods 6, 917–922. The HO-poly-KanMX4-HO plasmid was retrieved from: Voth, W.P., Richards, J.D., Shaw, J.M., and Stillman, D.J. (2001). Yeast vectors for integration at the HO locus. Nucleic Acids Res. 29, E59-9.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid can be used to assemble the auxin inducible degron in single yeast cells.
A characterization guide can be found here:
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOsTIR1w/oGFP was a gift from Matthias Heinemann (Addgene plasmid # 102883 ; http://n2t.net/addgene:102883 ; RRID:Addgene_102883)
For your References section:Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle. Papagiannakis A, Niebel B, Wit EC, Heinemann M. Mol Cell. 2017 Jan 19;65(2):285-295. doi: 10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15. 10.1016/j.molcel.2016.11.018 PubMed 27989441