Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle.
Papagiannakis A, Niebel B, Wit EC, Heinemann M
Mol Cell. 2017 Jan 19;65(2):285-295. doi: 10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15. PubMed Article

Plasmids from Article

ID Plasmid Purpose
92179pTEF:ATPHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
102882pG2JExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
102883pOsTIR1w/oGFPExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.
102884pG23APlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Antibodies from Article