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Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle.
Papagiannakis A, Niebel B, Wit EC, Heinemann M
Mol Cell. 2017 Jan 19;65(2):285-295. doi: 10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92179pTEF:ATPHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
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102882pG2JExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
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102883pOsTIR1w/oGFPExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.
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102884pG23APlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.
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