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pG23A
(Plasmid #102884)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102884 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HO-poly-KanMX4-HO
  • Backbone manufacturer
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/
  • Backbone size w/o insert (bp) 2531
  • Total vector size (bp) 5849
  • Vector type
    Yeast Expression
  • Selectable markers
    NatMX (select with Nourseotrichin)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc14/mCherry-AID(71-114)-NatMX
  • Alt name
    OAF3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2328
  • Entrez Gene
    CDC14 (a.k.a. YFR028C, OAF3)
  • Promoter Native promoter
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • AID(71-114) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGACGAATCCATGCAGG
  • 3′ sequencing primer ggtcacagcttgtctgtaa
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The AID(71-114) sequence was retrieved from: Morawska, M., and Ulrich, H.D. (2013). An expanded tool kit for the auxin-inducible degron system in budding yeast. Yeast 30, 341–351. mCherry was retrieved from the pBS35 plasmid.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.

A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6

Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG23A was a gift from Matthias Heinemann (Addgene plasmid # 102884 ; http://n2t.net/addgene:102884 ; RRID:Addgene_102884)
  • For your References section:

    Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle. Papagiannakis A, Niebel B, Wit EC, Heinemann M. Mol Cell. 2017 Jan 19;65(2):285-295. doi: 10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15. 10.1016/j.molcel.2016.11.018 PubMed 27989441