AT-U1
(Plasmid #112059)

• Purpose: U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)
• Depositing Lab: Amy Palmer
• Publication: Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038/s41589-018-0103-7. doi: 10.1038/s41589-018-0103-7.

Backbone

• Vector backbone: U1 human
• Backbone manufacturer: "Reduction of Target Gene Expression by a Modified U1 snRNA" (Beckley et al, 2001, Mol Cell Bio)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U1
• Alt name: NCBI Reference Sequence: NR_004430.2
• Species: H. sapiens (human)
• Mutation: 1 copy of Riboglow tag (truncated variant A) near 5' end of U1
• Entrez Gene: RNU1-1 (a.k.a. HSD1, HU1-1, RNU1, RNU1A, RNU1A3, RNU1G4, Rnu1a1, U1, U1A1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 3′ sequencing primer: U1-H-Seq_RV (5'-GGAAAGCGCGAACGCAGTC-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In partial sequence, 1 copy of Riboglow (truncated version A)--U1 sequence is in lower case letters, Riboglow sequence is in upper case letters