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PurposeControl for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin
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Depositing Lab
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Depositing OrganizationNational Center for Advancing Translational Sciences (NCATS)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 112085-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 112085-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep3xFLAG-CMV-10
- Backbone size w/o insert (bp) 6204
- Total vector size (bp) 8717
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3XFLAG firefly luciferase-beta-globin
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Alt nameFLuc NMD sensor control
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SpeciesH. sapiens (human), M. musculus (mouse); Photinus pyralis
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Insert Size (bp)2513
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Entrez GeneHBB (a.k.a. CD113t-C, ECYT6, beta-globin)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hgH-PA-R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byb-globin sequence used in this clone was obtained from Professor Lynne Maquat
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 112085-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 112085-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKC-4.04 was a gift from James Inglese (Addgene plasmid # 112085 ; http://n2t.net/addgene:112085 ; RRID:Addgene_112085) -
For your References section:
ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR. eLife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178. 10.7554/eLife.33178 PubMed 29528287
Map uploaded by the depositor.
Map uploaded by the depositor.
