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pEGFP-N1 Dynamin1 L330R Q334R L702R
(Plasmid #112105)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112105 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dynamin1
  • Species
    H. sapiens (human)
  • Mutation
    L330R Q334R L702R
  • Entrez Gene
    DNM1 (a.k.a. DEE31, DNM, EIEE31)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 Dynamin1 L330R Q334R L702R was a gift from Justin Taraska (Addgene plasmid # 112105 ; http://n2t.net/addgene:112105 ; RRID:Addgene_112105)
  • For your References section:

    Cryo-EM of the dynamin polymer assembled on lipid membrane. Kong L, Sochacki KA, Wang H, Fang S, Canagarajah B, Kehr AD, Rice WJ, Strub MP, Taraska JW, Hinshaw JE. Nature. 2018 Aug;560(7717):258-262. doi: 10.1038/s41586-018-0378-6. Epub 2018 Aug 1. 10.1038/s41586-018-0378-6 PubMed 30069048