CMV-mEGFP-PKCa-C1
(Plasmid
#112264)
-
PurposeITRACKalpha Donor Construct
-
Depositing Lab
-
Depositing OrganizationMax Planck Florida Institute
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 112264-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 112264-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonemEGFP-C1
- Backbone size w/o insert (bp) 4650
- Total vector size (bp) 6699
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameprotein kinase C alpha
-
Alt namePKCalpha
-
SpeciesB. taurus (bovine)
-
Insert Size (bp)2016
-
MutationA11V (please see depositor comments below)
-
GenBank IDAAI51472.1
-
Entrez GenePRKCA (a.k.a. PKRCA)
- Promoter CMV
-
Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GFP_C
- 3′ sequencing primer SV40
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation A11V was found in PKCa during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
DNA (Catalog # 112264-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 112264-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CMV-mEGFP-PKCa-C1 was a gift from Ryohei Yasuda (Addgene plasmid # 112264 ; http://n2t.net/addgene:112264 ; RRID:Addgene_112264) -
For your References section:
PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171
Map uploaded by the depositor.
