CMV-ShadowY-kCAAX
(Plasmid
#115564)
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Purposedark ITRACK acceptor construct for dual color imaging
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Depositing Lab
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Depositing OrganizationMax Planck Florida Institute
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 115564-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 115564-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 3200
- Total vector size (bp) 4044
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameShadowY with KRas membrane targeting domain
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Alt nameShY_Caax
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SpeciesSynthetic
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Insert Size (bp)810
- Promoter CMV
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Tag
/ Fusion Protein
- ShadowY (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Age1 (not destroyed)
- 3′ cloning site BAMHI (not destroyed)
- 5′ sequencing primer cmv
- 3′ sequencing primer SV40
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHideji Murakoshi _shadowY
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ShadowY developed by Hideji Murakoshi and Akihiro C. E. Shibata
DOI:10.1038/s41598-017-07002-4
DNA (Catalog # 115564-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 115564-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-ShadowY-kCAAX was a gift from Ryohei Yasuda (Addgene plasmid # 115564 ; http://n2t.net/addgene:115564 ; RRID:Addgene_115564) -
For your References section:
PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171
Map uploaded by the depositor.
