CMV-ShadowY-kCAAX
(Plasmid #115564)

• Purpose: dark ITRACK acceptor construct for dual color imaging
• Depositing Lab: Ryohei Yasuda
• Publication: Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 3200
• Total vector size (bp): 4044
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ShadowY with KRas membrane targeting domain
• Alt name: ShY_Caax
• Species: Synthetic
• Insert Size (bp): 810
• Promoter: CMV
• Tag / Fusion Protein:
  • ShadowY (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age1 (not destroyed)
• 3′ cloning site: BAMHI (not destroyed)
• 5′ sequencing primer: cmv
• 3′ sequencing primer: SV40

Resource Information

• Supplemental Documents:
  • CMVShadowY_CAAX.gb
• A portion of this plasmid was derived from a plasmid made by: Hideji Murakoshi _shadowY

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ShadowY developed by Hideji Murakoshi and Akihiro C. E. Shibata
DOI:10.1038/s41598-017-07002-4