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Addgene

pTXRE5-Z
(Plasmid #112509)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112509 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    unknown
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RW95-3 with 5x xenobiotic response elements
  • Alt name
    DRE
  • Alt name
    LacZ
  • Species
    Synthetic; E.coli
  • Entrez Gene
    lacZ (a.k.a. b0344, ECK0341)
  • Promoter 5 XREs+ minimal Cytochrome C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found that the LacZ translation lacks the last C-terminal 11 amino acids compared to NCBI reference sequence AAB49976.1. The depositing laboratory states that this difference does not adversely affect protein function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXRE5-Z was a gift from Charles Miller (Addgene plasmid # 112509 ; http://n2t.net/addgene:112509 ; RRID:Addgene_112509)
  • For your References section:

    A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures. Miller CA 3rd. Toxicol Appl Pharmacol. 1999 Nov 1;160(3):297-303. doi: 10.1006/taap.1999.8769. 10.1006/taap.1999.8769 PubMed 10544064