Skip to main content
Addgene
Open Menu

pCAG-VHC
(Plasmid #112618)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112618 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 112618-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 112618-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    backbone from pd2EGFP-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3110
  • Total vector size (bp) 9852
  • Modifications to backbone
    CAG promoter inserted at MCS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    H2B-Venus
  • Alt name
    HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    1101
  • GenBank ID
    NM_021058
  • Entrez Gene
    H2BC3 (a.k.a. H2B.1, H2B/f, H2BFF, HIST1H2BB)
  • Promoter CAG
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAAGAATTCATGAGGCCGGCC
  • 3′ sequencing primer TGAAGTTAGTAGCTCCGCTTCC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Hygrophosphotransferase
  • Alt name
    HygroR
  • Alt name
    hygromycin-B
  • Species
    Coccidioides posadasii C735 delta SOWgp
  • Insert Size (bp)
    1023
  • GenBank ID
    XM_003071606
  • Promoter CAG

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atgaaaaagcctgaactcaccgc
  • 3′ sequencing primer ttcctttgccctcggacga
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Cre-ERT2 fusion protein
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    1983
  • Promoter CAG

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGTCCAATTTACTGACCGTACA
  • 3′ sequencing primer TCAAGCTGTGGCAGGGAAACC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H2B-Venus was cloned from Addgene plasmid 26750, HygroR was cloned from Addgene plasmid 28226, CreERT2 and Rabbit betaglobin PolyA were cloned from Addgene plasmid 14797, the CAG promoter was cloned from Addgene plasmid 14757

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CAG promoter driving multicistronic reporter plasmid containing H2BVenus, HygroR, and CreERT2 in a single ORF with two P2A sequences.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-VHC was a gift from Stefan Heller (Addgene plasmid # 112618 ; http://n2t.net/addgene:112618 ; RRID:Addgene_112618)

  • For your References section:

    Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, Heller S. Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 10.1016/j.ydbio.2018.08.013. 10.1016/j.ydbio.2018.08.013 PubMed 30179592
Related items:
Commonly requested with: