DRH-307
(Plasmid #112670)

• Purpose: Lentiviral expression of GFP under the CAMK2A promoter in mammalian cells
• Depositing Lab: Adam Cohen
• Publication: Nehme et al Cell Rep. 2018 May 22;23(8):2509-2523. doi: 10.1016/j.celrep.2018.04.066.

Backbone

• Vector backbone: unknown
  (Search Vector Database)
• Backbone manufacturer: DHR
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 9988
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 1292
• Promoter: CAMK2A
• Tag / Fusion Protein:
  • eGFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Deposited on behalf of Adam Cohen and Daniel Hauchbaum