pCI-Neo-JAM-A
(Plasmid
#112965)
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PurposeTo express feline Jam-A transiently in cell lines that do not naturally express the receptor.
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Depositing Lab
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Depositing OrganizationCornell University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 112965-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 112965-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePCI-Neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameJunctional Adhesion Molecule type A (JAM-A)
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SpeciesFelis catus
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GenBank ID
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Entrez GeneF11R (a.k.a. JAM-1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 112965-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 112965-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Neo-JAM-A was a gift from John S. L. Parker (Addgene plasmid # 112965 ; http://n2t.net/addgene:112965 ; RRID:Addgene_112965) -
For your References section:
Conserved Surface Residues on the Feline Calicivirus Capsid Are Essential for Interaction with Its Receptor Feline Junctional Adhesion Molecule A (fJAM-A). Lu Z, Ledgerwood ED, Hinchman MM, Dick R, Parker JSL. J Virol. 2018 Mar 28;92(8):e00035-18. doi: 10.1128/JVI.00035-18. Print 2018 Apr 15. 10.1128/JVI.00035-18 PubMed 29386293
