PAK4-FL-3'UTR
(Plasmid
#113088)
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PurposeLuciferase reporter containing PAK4- FL-3'UTR
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Depositing Lab
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Depositing OrganizationVA Medical Center - Baltimore
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 113088-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 113088-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmirGLO Dual-Luciferase
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 7350
- Total vector size (bp) 8140
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep21 Activated kinase 4
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Alt namePAK4
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SpeciesH. sapiens (human)
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Insert Size (bp)790
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Mutationnone
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GenBank IDNM_001014834.2 NM_001014834.2
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Entrez GenePAK4
- Promoter PGK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Dra I (not destroyed)
- 3′ cloning site Sac I (not destroyed)
- 5′ sequencing primer ACACGGTAAAACCATGAC
- 3′ sequencing primer GTCCAAACTCATCAATGTA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 113088-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 113088-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAK4-FL-3'UTR was a gift from James Donahue (Addgene plasmid # 113088 ; http://n2t.net/addgene:113088 ; RRID:Addgene_113088) -
For your References section:
MiR-199a-3p decreases esophageal cancer cell proliferation by targeting p21 activated kinase 4. Phatak P, Burrows WM, Chesnick IE, Tulapurkar ME, Rao JN, Turner DJ, Hamburger AW, Wang JY, Donahue JM. Oncotarget. 2018 Jun 19;9(47):28391-28407. doi: 10.18632/oncotarget.25375. eCollection 2018 Jun 19. 10.18632/oncotarget.25375 PubMed 29983868
