PAK4-FL-3'UTR-MT-both
(Plasmid #113094)

• Purpose: Luciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutated
• Depositing Lab: James Donahue
• Publication: Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-28407. doi: 10.18632/oncotarget.25375. eCollection 2018 Jun 19.

Backbone

• Vector backbone: pmirGLO Dual-Luciferase
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 7350
• Total vector size (bp): 8139
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p21 Activated kinase 4
• Alt name: PAK4
• Species: H. sapiens (human)
• Insert Size (bp): 790
• Mutation: Both the predicted miR-199a-3p binding site mutated by substitution of 4 bases
• GenBank ID: NM_001014834.2 NM_001014834.2
• Entrez Gene: PAK4
• Promoter: PGK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: DraI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: ACACGGTAAAACCATGAC
• 3′ sequencing primer: GTCCAAACTCATCAATGTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry