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pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
(Plasmid #113686)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113686 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Alligent Technologies
  • Total vector size (bp) 7043
  • Vector type
    AAV, Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    de-catalyzed SaCas9
  • Species
    Synthetic
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan Weissman, pHR-SFFV-dCas9-BFP-KRAB plasmid #46911, Cre-Lox sites from Karl Deisseroth, pAAV-Ef1a-DIO EYFP plasmid #27056
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA was a gift from Jonathan Ploski (Addgene plasmid # 113686 ; http://n2t.net/addgene:113686 ; RRID:Addgene_113686)
  • For your References section:

    The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Kumar N, Stanford W, Aradhana CS, Abraham ND, Dao TJ, Thaseen S, Sairavi A, Gonzalez CU and Ploski JE.. Front. Mol. Neurosci. (2018) 13 10.3389/fnmol.2018.00413