|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113686||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAlligent Technologies
- Total vector size (bp) 7043
Vector typeAAV, Cre/Lox, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namede-catalyzed SaCas9
/ Fusion Protein
- KRAB (C terminal on insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA was a gift from Jonathan Ploski (Addgene plasmid # 113686 ; http://n2t.net/addgene:113686 ; RRID:Addgene_113686)
For your References section:The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Kumar N, Stanford W, Aradhana CS, Abraham ND, Dao TJ, Thaseen S, Sairavi A, Gonzalez CU and Ploski JE.. Front. Mol. Neurosci. (2018) 13 10.3389/fnmol.2018.00413