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pGEM-gate
(Plasmid #113758)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113758 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3048
  • Vector type
    Gateway Destination Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    May use DB3.1 cells instead of ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gateway cassette
  • Insert Size (bp)
    1703

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-gate was a gift from Magdalena Bezanilla (Addgene plasmid # 113758 ; http://n2t.net/addgene:113758 ; RRID:Addgene_113758)
  • For your References section:

    Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Mallett DR, Chang M, Cheng X, Bezanilla M. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. 10.1002/pld3.168 PubMed 31523744