Crimson/P2A-NLS/HA-HumBeta (pSLIK2)
(Plasmid #113857)

• Purpose: For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene
• Depositing Lab: Richard S Myers
• Publication: Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. doi: 10.1371/journal.pone.0200955. eCollection 2018.

Backbone

• Vector backbone: pSLIK-Zeo
• Backbone manufacturer: Iain Fraser (Addgene plasmid # 25736)
• Modifications to backbone: The Synaptase gene is fused to E2-Crimson fluorescent protein gene through a P2A linker to follow synaptase expression without tethering E2-Crimson to the synaptase protein. The bacteriophage lambda Beta gene was redesigned to be better expressed in human cells and fused to a NLS and an HA epitope tag.
• Vector type: Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Humanized Beta
• Species: Synthetic
• Mutation: "humanized" Beta gene
• Tag / Fusion Protein:
  • P2A/Crimson (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/02/11/259739 for bioRxiv preprint.