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(Plasmid #113888)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113888 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Vector type
  • Selectable markers
  • Tag / Fusion Protein
    • 3C-Avi-His6 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Promoter CMV-MIE-TetO2
  • Tag / Fusion Protein
    • 3C-Avi-His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, AgeI (not destroyed)
  • 3′ cloning site KpnI, XhoI (not destroyed)
  • 5′ sequencing primer CGTCGTCGTGCTCGTTTAGTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This empty backbone contains a 12bp dummy insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP was a gift from A. Radu Aricescu (Addgene plasmid # 113888 ; ; RRID:Addgene_113888)
  • For your References section:

    Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Elegheert J, Behiels E, Bishop B, Scott S, Woolley RE, Griffiths SC, Byrne EFX, Chang VT, Stuart DI, Jones EY, Siebold C, Aricescu AR. Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 10.1038/s41596-018-0075-9. 10.1038/s41596-018-0075-9 PubMed 30455477