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pEBTet_NAD-Snifit-cyto
(Plasmid #113914)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113914 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEBTet
  • Backbone size w/o insert (bp) 11448
  • Total vector size (bp) 13797
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NAD-Snifit
  • Alt name
    hSPR(D41W42)-HaloTag7-Pro30-SNAPtag
  • Species
    Synthetic
  • Insert Size (bp)
    2349
  • Promoter CMV TetO2

Cloning Information

Resource Information

Depositor Comments

Plasmid contains some differences in the oriP backbone sequence compared to the depositor sequence. These differences are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBTet_NAD-Snifit-cyto was a gift from Kai Johnsson (Addgene plasmid # 113914 ; http://n2t.net/addgene:113914 ; RRID:Addgene_113914)
  • For your References section:

    Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 32638 [pii] PubMed 29809136