pDEST-FRT/TO-eGFP-REV7
(Plasmid
#114128)
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PurposeExpresses N-terminally tagged eGFP-REV7 in mammalian cells
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Depositing Lab
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Depositing OrganizationSinai Health System
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 114128-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 114128-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepDEST-FRT/TO-eGFP
- Backbone size w/o insert (bp) 5864
- Total vector size (bp) 6497
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Vector typeMammalian Expression ; Genomic integration, tetracycline inducible
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameREV7
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SpeciesH. sapiens (human)
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Insert Size (bp)633
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Entrez GeneMAD2L2 (a.k.a. FANCV, MAD2B, POLZ2, REV7)
- Promoter CMV/TetO2
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Tag
/ Fusion Protein
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer BGH Reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byORFeome
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 114128-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 114128-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-FRT/TO-eGFP-REV7 was a gift from Daniel Durocher (Addgene plasmid # 114128 ; http://n2t.net/addgene:114128 ; RRID:Addgene_114128) -
For your References section:
The shieldin complex mediates 53BP1-dependent DNA repair. Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18. 10.1038/s41586-018-0340-7 PubMed 30022168
